Micropipette Principle: A Comprehensive Overview of Accurate Liquid Measurement

Micropipette principle is essential for anyone working in laboratories, research, and academic environments that demand precision in liquid measurement. These versatile instruments are designed to measure and transfer small volumes of liquid, typically ranging from 0.1 µL to 1000 µL. Understanding the micropipette principle is crucial for achieving accuracy and reproducibility in experiments.

Micropipettes are essential tools in scientific research, allowing for precise and accurate measurement and transfer of small volumes of liquid. They are commonly used in fields such as biology, chemistry, and medicine, where the accuracy of measurements can have a significant impact on research outcomes. In this article, we will explore the principle of micropipettes and how they work.

The principle behind the function of a micropipette is based on air displacement. The micropipette consists of a piston, a calibrated volume, and a tip. When the piston is pushed down, it creates a vacuum that draws the liquid into the tip. The volume of the liquid drawn into the tip is determined by the calibrated volume of the micropipette. When the piston is released, the liquid is dispensed from the tip.

Micropipettes come in different sizes, with the most commonly used being the P10, P20, P200, and P1000 micropipettes. The numbers correspond to the maximum volume that can be dispensed by the micropipette, in microliters (µL).

To use a micropipette, the user must first set the desired volume on the micropipette using the volume adjustment dial. The micropipette is then used to aspirate the liquid by placing the tip into the liquid and depressing the plunger to the first stop. The tip should be fully submerged in the liquid to prevent air bubbles from being drawn into the tip. Once the liquid has been drawn into the tip, the micropipette is removed from the liquid, and the tip is wiped on the side of the container to remove any excess liquid.

To dispense the liquid, the tip is placed into the receiving vessel, and the plunger is depressed to the second stop. It is important to fully depress the plunger to ensure that all of the liquid is dispensed from the tip. Once the liquid has been dispensed, the tip is ejected by pressing the tip ejector button.

In conclusion, the principle of micropipettes is based on air displacement, and they are used to accurately measure and transfer small volumes of liquid. Understanding how to use a micropipette correctly is essential to ensure accurate results in scientific research.

Product Specification

Features :

• 10-100ul Micropipette
• QC Test Report
• Aquepis Samples
• Vasoactive 
• Multi Color 

Product details

• Best Sellers Rank: #5,235 in Industrial & Scientific (See Top 100 in Industrial & Scientific)

micropipettes,Product Description

Micropipette Excellent Variable Volume Eight volume ranges from 0.5 µl to 10000 µl let you select the right pipette for your application by follwing ways:

• Easy volume setting by simply turning the plunger.
• Click stop digital system
• Click sound at each increment during volume setting ensures no unwanted volume alterations during pipetting.
• Separate, streamlined tip ejector designed for access to narrow necked containers and tubes.
• Easy to calibrate and service.
• Autoclavable tip cone and lower section assembly.

Variable Volume semi autoclavable  Micropipette is a pipette that combines all the basics of a great device. A pipette is a lab device typically utilized in chemistry, biology as well as medicine to dispense a measured quantity of liquids like reagents, acids, alkalies, and media. Pipettes can be found in numerous layouts or designs for multiple functions with varying degrees of accuracy and precision, right from single piece glass pipettes to more complicated adjustable, flexible micro or electronic pipettes suitable for a wide range of experiments.

Features of a  Variable Volume semi autoclavable Micropipette:

• high accuracy,
• lightweight
• long durability

Micropipettes operate on a basic principle that involves the creation of a vacuum to draw up liquid into a disposable tip. The device has a plunger that, when pressed down, expels air, creating a vacuum. Once the plunger is released, the vacuum pulls liquid into the tip. This mechanism allows for controlled measurement of liquids, making micropipettes indispensable in various scientific fields.

To effectively utilize a micropipette, it is essential to understand its components. A micropipette generally consists of a body, a plunger, a volume setting mechanism, and disposable tips. The body houses the internal mechanisms and the plunger, which creates the suction force. The volume setting mechanism allows users to adjust the volume of liquid they wish to measure, ensuring flexibility in liquid handling.

One of the key aspects to consider when working with the micropipette principle is the selection of appropriate pipette tips. Tips come in different sizes and volumes, and using the right size is crucial for accurate measurements. Moreover, it's vital to use high-quality tips to prevent contamination and ensure that the liquid is not retained in the tip after dispensing.

Error minimization is another critical factor that adheres to the micropipette principle. Practice proper pipetting technique by holding the micropipette vertically, immersing the tip into the liquid to an appropriate depth, and avoiding excessive force on the plunger. Furthermore, it’s advisable to eject tips with the plunger fully depressed to prevent liquid from clinging to the tip.

Regular calibration and maintenance of micropipettes are fundamental to uphold their accuracy. Over time, pipettes can drift from their calibrated settings, causing inaccuracies in measurements. Therefore, routine checks should be conducted to verify that the micropipette's volume measurements align with the expected values. Many laboratories implement a calibration schedule as part of their standard operating procedures.

Another aspect of the micropipette principle involves understanding the importance of temperature and viscosity. The viscosity of the liquid being measured can affect how the liquid moves within the micropipette. Adjusting pipetting techniques based on liquid properties will enhance measurement accuracy. Additionally, conducting pipetting operations within a controlled environment can minimize the impact of temperature fluctuations.

After mastering the micropipette principle, researchers can apply it toward various applications. From molecular biology tasks such as PCR and DNA quantification to routine laboratory procedures like sample dilutions and cell culture, micropipettes are integral to scientific discovery. The versatility of these instruments allows users across diverse scientific disciplines to achieve consistent and reliable results.

In conclusion, understanding the micropipette principle ensures accurate liquid measurement, essential for conducting successful experiments. By grasping the mechanics of micropipettes, adhering to proper techniques, and maintaining regular calibration, users can enhance their laboratory practices significantly. Whether you are an experienced researcher or new to the field, having a strong foundation in the micropipette principle will empower you to conduct precise experiments that yield dependable data.